Smartox/Maurotoxin Kv and SK channels blocker/08MAR001-00500/0.5mg,Smartox,,Smartox,smartox-biotech,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Smartox/Maurotoxin Kv and SK channels blocker/08MAR001-00500/0.5mg

产品编号：08MAR001-00500

市场价：¥8736.00

场地：美国(厂家直采)

产品分类：生化试剂>缓冲液>酸碱缓冲液>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$840.00

品牌： Smartox

公司：Smartox

公司分类：

商品介绍

Maurotoxin isacomponentofthevenomofScorpiomauruspalmatus. Maurotoxin isamemberoftheα-KTx6.2scorpiontoxinfamily.Itblocksvoltage-gatedpotassiumchannels(K_V1.1/KCNA1,K_V1.2/KCNA2,andK_V1.3/KCNA3)andinhibitsapamin-sensitivesmallconductancecalcium-activatedchannels(SKchannels),particularlyK_Ca3.1(IKca1,SK4).TheblockageofK_v1.2occurswithhighaffinity.

Description:

Productcode:N/A.Categories:Kvchannels,Potassiumchannels.Tags:kv,SK.

AAsequence: Val-Ser-Cys³-Thr-Gly-Ser-Lys-Asp-Cys⁹-Tyr-Ala-Pro-Cys¹³-Arg-Lys-Gln-Thr-Gly-Cys¹⁹-Pro-Asn-Ala-Lys-Cys²⁴-Ile-Asn-Lys-Ser-Cys²⁹-Lys-Cys³¹-Tyr-Gly-Cys³⁴-NH₂Disulfidebonds: Cys³-Cys²⁴,Cys⁹-Cys²⁹,Cys¹³-Cys¹⁹andCys³¹ -Cys³⁴
Length(aa): 34
Formula: C₁₄₅H₂₃₁N₄₅O₄₇S₈MolecularWeight: 3612.55Da
Appearance:Whitelyophilizedsolid
Solubility: waterorsalinebuffer
CASnumber: notavailable
Source: Synthetic
Purityrate: >95%

Reference:

Analysisoftheinteractingsurfaceofmaurotoxinwiththevoltage-gatedShakerBK(+)channel

Maurotoxin(MTX)isa34-residuetoxinthatwasisolatedinitiallyfromthevenomofthescorpionScorpiomauruspalmatus.Unliketheothertoxinsoftheα-KTx6family(Pi1,Pi4,Pi7,andHsTx1),MTXexhibitsauniquedisulfidebridgeorganizationofthetypeC(1)C(5),C(2)C(6),C(3)C(4),andC(7)C(8)(insteadoftheconventionalC(1)C(5),C(2)C(6),C(3)C(7),andC(4)C(8),hereinreferredtoasPi1-like)thatdoesnotpreventitsfoldingalongtheclassicα/βscaffoldofscorpiontoxins.MTX(Pi1)isanMTXvariantwithaconventionalpatternofdisulfidebridgingwithoutanyprimarystructurealterationofthetoxin.Here,usingMTXand/orMTX(Pi1)asmodels,weinvestigatedhowthetypeoffoldinginfluencestoxinrecognitionoftheShakerBpotassiumchannel.AminoacidresiduesofMTXthatwerestudiedforShakerBrecognitionwereselectedonthebasisoftheirhomologouspositionincharyBDotoxin,athreedisulfide-bridgedscorpiontoxinalsoactiveonthischanneltype.TheseresiduesfavoredeitheranMTX-orMTX(Pi1)-likefolding.OurdataindicateclearlythatLys(23)andTyr(32)(twooutoftenaminoacidresiduesstudied)arethemostimportantresiduesforShakerBchannelblockagebyMTX.ForactivityonSKCachannels,thesameaminoacidresiduesalsoaffect,directlyorindirectly,therecognitionofSKchannels.ThemolecularmodelingtechniqueandcomputeddockingindicatetheexistenceofacorrelationbetweenthehalfcystinepairingsofthemutatedanalogsandtheiractivityontheShakerBK(+)channel.Overall,mutationsinMTXcould,orcouldnot,changethereorganizationofdisulfidebridgesofthismoleculewithoutaffectingitsα/βscaffold.However,changingofthepeptidebackbone(crosslinkingdisulfidebridgesfromMTX-liketypevsMTX(Pi1)-liketype)appearstohavelessimpactonthemoleculeactivitythanmutationofcertainkeyaminoacidssuchasLys(23)andTyr(32)inthistoxin.FajlounZ, etal.,(2011)Analysisoftheinteractingsurfaceofmaurotoxinwiththevoltage-gatedShakerBK(+)channel. JPeptSci. PMID: 21308876

Effectofmaurotoxin,afourdisulfide-bridgedtoxinfromthechactoidscorpionScorpiomaurus,onShakerK+channels

Maurotoxinisa34-residuetoxinisolatedfromthevenomoftheTunisianchactoidscorpionScorpiomauruspalmatusandcontainsfourdisulfidebridgesthatarenormallyfoundinlong-chaintoxinsof60-70aminoacidresidues,whichaffectvoltage-gatedsodiumchannels.However,despitetheunconventionaldisulfide-bridgepatternofmaurotoxin,theconformationofthistoxinremainssimilartothatofothertoxinsactingonpotassiumchannels.Here,weanalyzedtheeffectsofsyntheticmaurotoxinonvoltage-gatedShakerpotassiumchannels(ShB)expressedinXenopusoocytes.Maurotoxinproducesastrong,butreversIBLe,inhibitionoftheShBK+currentwithanIC50of2nM.Increasingconcentrationsofthetoxininduceaprogressivelyhigherblockatsaturatingconcentrations.Atnonsaturatingconcentrationsofthetoxin(5-20nM),thechannelblockappearsslightlymorepronouncedatthresholdpotentialssuggestingthatthetoxinmayhaveahigheraffinityfortheclosedstateofthechannel.Atthesinglechannellevel,thetoxindoesnotmodifytheunitarycurrentamplitude,butdecreasesensemblecurrentsbyincreasingthenumberofdepolarizingepochsthatfailedtoelicitanyopening.ApointmutationofLys23toalanineinmaurotoxinproducesa1000-foldreductionintheIC50ofblockbythetoxinsuggestingtheimportanceofthischargedresiduefortheinteractionwiththechannel.MaurotoxindoesnotaffectK+currentscarriedbyKir2.3channelsinoocytesorNa+currentscarriedbythealphaIIachannelexpressedinCHOcells.Carlier,E.,etal.(2000)Effectofmaurotoxin,afourdisulfide-bridgedtoxinfromthechactoidscorpionScorpiomaurus,onShakerK+channels, JPept. PMID: 10888198

Maurotoxin,afourdisulfidebridgesscorpiontoxinactingonK+channels

Maurotoxin,a toxin fromthevenomoftheTunisianchactoid scorpion Scorpiomaurus,hasbeenpurifiedtohomogeneitybygelfiltration/reversed-phaseHPLC,andcharacterized.ItisabasicandC-terminalamidated34-residuepolypeptidecross-linkedby four disulfide bridges.FromEdmansequencingresults,onlysixdifferentpairingsbetweenthefirstsixhalf-cystineswereretainedwhereasa disulfide bridgewaspredictedbetweenthetwohalf-cystinesinpositions31and34.Modellingbasedonthestructureofcharybdotoxinfavoredtwodifferentpairings,oneofwhichpossessedtwodisulfidesincommonwiththegeneralmotifof scorpion toxins.Thesolid-phasetechniquewasusedtoobtainsynthetic maurotoxin,sMTX.Thehalf-cystinepairingsofsMTXweredeterminedbyenzymaticcleavageandwerefoundtobeCys3Cys24,Cys9-Cys29,Cys13-Cys19,andCys31-34,inagreementwithexperimentaldataobtainedwithnatural maurotoxin.Bothnaturalandsyntheticmaurotoxinswerelethaltomicefollowingintracerebroventricularinjection(LD50,80ng/mouse).TheyblockedtheKv1.1,Kv1.2,andKv1.3 channels expressedinXenopusoocyteswithalmostidenticalhalf-effects(IC50)intherangeof40,0.8and150nM,respectively.Theyalsocompetedwith125I-apamin(SKcachannelblocker)and125I-kaliotoxin(Kvchannelblocker)forbindingtoratbrainsynaptosomeswithIC50ofabout5and0.03nM.Asthenaturalandsyntheticmaurotoxinsexhibitindistinguishablephysicochemicalandpharmacologicalproperties,theyarelikelytoadoptthesamehalf-cystinepairingpatternwhichisuniqueamongknown scorpion toxins.However,this disulfide organizationisdifferentfromthosereportedforPandinusimperatorandHeterometrusspinnifertoxins1(Pi1andHsTx1),twonovel four-disulfide bridged K+channel-acting scorpion toxin sharingabout50-70%sequenceidentitywith maurotoxin.

Rochat,H., etal.(1998)Maurotoxin,afourdisulfidebridgesscorpiontoxinactingonK+channels, Toxicon.PMID: 9792177

Solutionstructureofmaurotoxin,ascorpiontoxinfromScorpiomaurus,withhighaffinityforvoltage-gatedpotassiumchannels

Maurotoxin (MTX),purifiedfromthescorpionid Scorpio maurus isapotentligandfor potassium channels.ItshowsabroadspecificityasbeingactiveonKv1.1(Kd=37nM),Kv1.2(Kd=0.8nM),Kv1.3(Kd=150nM) voltage-gated potassium channels,aswellasonsmall-conductancecalcium-activated potassium channels.Ithasauniquedisulfidepairingamongthe scorpion toxinsfamily.The solution structure ofMTXhasbeendeterminedby2D-NMRtechniques,whichledtothefulldescriptionofits3Dconformation:abendedhelixfromresidues6to16connectedbyalooptoatwo-strandedantiparallelbetasheet(residues23to26and28to31).TheinteractionofMTXwiththeporeregionoftheKv1.2 potassium channelhasbeenmodeledaccordingtotheirchargeanisotropy.The structure ofMTXissimilartoothershort scorpion toxinsdespiteitspeculiardisulfidepairing.ItsinteractionwiththeKv1.2channelinvolvesadipolemoment,whichguidesandorientsthe toxin ontothepore,towardthebindingsite,andwhichthusisresponsibleforthespecificity.

Blanc,E., etal.(1997)Solutionstructureofmaurotoxin,ascorpiontoxinfromScorpiomaurus,withhighaffinityforvoltage-gatedpotassiumchannels, Proteins. PMID: 9365987

品牌介绍

Smartox Biotechnolgy的多肽毒素产品如下：

1. 作用于钠离子通道(Sodium channel)的毒素

Toxin name	Catalog #	Target
Phrixotoxin-3	13PHX003	Selective blocker of Nav1.2
µ-conotoxin GIIIB	CON020	Selective blocker of Nav1.4
µ-conotoxin CnIIIC	CON021	Selective blocker of Nav1.4
μ-conotoxin PIIIA	08CON006	Selective blocker of Nav1.4
Jingzhaotoxin-III	12JZH003	Selective blocker of Nav1.5
ProTx-II	07PTX002	Selective blocker of Nav1.7
ProTx-II Biotin	12PTB002	Selective blocker of Nav1.7
ProTx-I	12PTX001	Blocker of Nav1.8, Nav1.2, Nav1.5, Nav1.7
Huwentoxin-I	07HWT001	Blocker of TTX-S
Huwentoxin-IV	08HWT002	Blocker of TTX-S
Hainantoxin-III	13HTX003	Blocker of TTX-S
Hainantoxin-IV	12HTX001	Blocker of TTX-S
GsAF-I	12GSF001	Blocker of TTX-S
GsAF-II	12GSF002	Blocker of TTX-S

2. 作用于钾离子通道(Potassium channel)的毒素

Toxin name	Catalog #	Target
K_Ca channels
Apamin 蜜蜂神经毒素	08APA001	SK1, SK2, SK3
Charybdotoxin 蝎毒素	11CHA001	KCa1.1, KCa3.1 - Kv1.2, Kv1.3, Kv1.6
Iberiotoxin	12IBX001	KCa1.1
Leiurotoxin 1 (Scyllatoxin)	10LEI001	SK1, SK2, SK3
Tamapin	10TAM001	SK1, SK2, SK3
Kaliotoxin-1	08KTX002	BK, Kv1.1, Kv1.2, Kv1.3
Kv channels
ShK	08SHK001	Kv1.3, Kv1.1, Kv1.4, Kv1.6
TMR-ShK	SAT001	Kv1.3, Kv1.1
Margatoxin	08MAG001	Kv1.3
(Dap22)-ShK	13SHD001	Kv1.3
ADWX-1	13ADW001	Kv1.3
HsTx1	08NEU001	Kv1.3, Kv1.2
Agitoxin-2	13AGI002	Kv1.3, Kv1.1
Maurotoxin	08MAR001	Kv1.2, KCa3.1
Guangxitoxin 1E	11GUA002	Kv2.1, Kv2.2
Stromatoxin 1 NEW	SCT01	Kv2.1, Kv2.2
Kaliotoxin-1	08KTX002	BK, Kv1.1, Kv1.2, Kv1.3
Charybdotoxin	11CHA001	KCa1.1, KCa3.1 - Kv1.2, Kv1.3, Kv1.6
Phrixotoxin-2	PHX002	Kv4.2, Kv4.3
AmmTx3 NEW	AMX001	A-type potassium channels
Inwardly rectifying potassium channels
TertiapinQ	08TER001	Kir1.1, Kir3.1/3.4, Kir3.1/3.2-KCa1.1
hERG/Kv11.1
BeKm-1	13BEK001	ERG1

3. 作用于钙离子通道(Calcium channel)的毒素

Toxin name	Catalog #	Target
High voltage-gated Ca²⁺ channels
ω-agatoxin IVA	11AGA001	P/Qtype
ω-Conotoxin MVIIC	08CON002	P/Qtype, N-type
ω-Conotoxin MVIIA	08CON001	N-type
ω-Conotoxin GVIA	08CON003	N-type
ω-Conotoxin SO3	08CON013	N-type
Huwentoxin I	07HWT001	N-type
ProTx-II	07PTX002	T-type, L-type
Intermediate voltage-gated Ca²⁺ channels
SNX482	08SNX002	R-type
Low voltage-gated Ca²⁺ channels
ProTx-I	12PTX001	T-type
ProTx-II	07PTX002	T-type, L-type
Ryanodine receptors
Maurocalcine	07PAU001	Ryr1

4. 作用于氯离子通道(Chloride channel)的毒素

Toxin name	Catalog #	Target
Chlorotoxin	08CHL001	Blocker of small conductance Cl- channels
GaTx1	13GTX001	Selective blocker of CFTR channel
GaTx2	10GTX002	Selective blocker of ClC-2 channel

5. 作用于乙酰胆碱受体(Acetylcholine receptor)的毒素

Toxin name	Catalog #	Target
α-conotoxin PeIA	13CON017	α9α10, α3β2 subunits
α-Conotoxin PrXA	13CON016	α1/β1/ε/δ, α1/β1/γ/δ subunits
Waglerin-1	12WAG001	MusclenAChR
α-conotoxin MI	08CON012	α1/δsubunits
α-conotoxin GI	08CON005	α/δsite
α-conotoxin IMI	08CON011	α7 homomeric nAChR
α-conotoxin GID	CON019	Blocker of α3β2, α7 and α4β2 nAChRs

6. 含N-甲基-D-天冬氨酸NR2B

(NMDA, NR2B containing N-methyl-D-aspartate)

Conantokin-G

选择性、特异性抑制含NR2B的NMDAR。Conantokin-G能剂量依赖性抑制Ca2+内流，抑制NMDA诱导的兴奋性中毒效应。研究表明，在小鼠皮层神经元，Conantokin-G阻滞NMDA引发的电流信号的IC50值为480 nM。

7. 作用于酸敏感离子通道(ASIC channel, Acid-Sensing Ion Channel)的毒素

Toxin name	Catalog #	Target
APETx2	07APE002	Selective blocker of ASIC3
Psalmotoxin1/PcTx1	13PCT001	Selective blocker of ASIC1a
Ugr9-1	13UGR001	Blocker of ASIC3

8. 作用于瞬时受体电位(TRP channel, transient receptor potential)的毒素

Toxin name	Catalog #	Target
GsMTx4	08GSM001	TRPC, TRPA
Vanillotoxin3	10VAN003	Activator of TRPV1
ProTx-I	12PTX001	Antagonist of TRPA1

9. 作用于嘌呤能通道(Purinergic channel)的毒素

Purotoxin-1

选择性抑制P2X3受体。100 nM Purotoxin-1 (PT-1)选择性抑制P2X3受体通道，在大鼠DRG神经元上，使用膜片钳实验表明：PT-1对电压门控通道和TRPV1均无抑制效应。10 µM ATP和100 µM α,β Methylene-ATP浓度下Purotoxin-1对P2X3受体有选择性作用，在该ATP浓度下Purotoxin-1对P2X2和杂化二聚体P2X2/3并无激动作用。Purotoxin-1对疼痛的潜在治疗作用。

10. 作用于其它膜受体通道(Others)的毒素

Smartox Biotechnology还提供其他类型的膜受体抑制剂：

Toxin name	Catalog #	Target
Morphiceptin	011CAS001	Agonist of µ-opoid receptors
Lys-conopressin G	11CON14	Vasopressin-like peptide
GsMTx4	08GSM001	Mechano sensitive ion channels
Obtustatin	10OBT001	Blocks the binding of α1β1 integrin to collagen IV
Rho-Conotoxin TIA	CON022	Blocks α1-adrenergic receptor

公司简介

Smartox Biotechnology是全球唯一一家专门生产动物毒液多肽毒素，用于细胞离子通道功能研究的生物医药公司。多肽毒素在生物制药领域具有重要的使用价值。

Smartox Biotechnology于2009年由来自Grenoble神经科学研究所(Grenoble Institute of Neuroscience)的Michel De Waard博士创立。Smartox Biotechnology专门研究动物毒液，制作合成多种毒液中的多肽成分（常称为毒素）。De Waard博士研究离子通道与毒素多肽的关系，尤其是鉴定、开发毒素多肽作为治疗性分子或细胞穿透肽(cell penetrating peptides, CPP)。其研究团队在毒液分离，药理性活性肽鉴定、富半胱氨酸肽定性、制作和优化等方面具有独特、丰富的经验。2010年，Smartox Biotechnolgy被法国研究部(Ministry of Research)授予“新兴企业OSEO奖(OSEO prize for emerging businesses)”。

总之，Smartox Biotechnolgy提供一系列高质量、具开创价值的多肽毒素。这些化合物在离子通道研究中具有高的亲和性和选择性，是相应领域科学研究理想的生物毒素提供商和贴心的合作伙伴。

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